The potential of mannosylated chitosan microspheres to target macrophage mannose receptors in an adjuvant-delivery system for intranasal immunization
✍ Scribed by Hu-Lin Jiang; Mi Lan Kang; Ji-Shan Quan; Sang Gyun Kang; Toshihiro Akaike; Han Sang Yoo; Chong-Su Cho
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 905 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0142-9612
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✦ Synopsis
A vaccine delivery system based on mannosylated chitosan microspheres (MCMs) was studied in vitro and in vivo. Bordetella bronchiseptica antigens containing dermonecrotoxin (BBD) were loaded in MCMs or chitosan microspheres (CMs). Fluorescence confocal microscopy indicated that BBD-loaded MCMs (BBDeMCMs) bound with mannose receptors on murine macrophages (RAW264.7 cells). In vitro experiments using macrophages demonstrated that BBDeMCMs had more effective immune-stimulating activity than BBD-loaded CMs (BBDeCMs). Mice intranasally immunized with BBDeMCMs showed significantly higher BBD-specific IgA antibody responses in saliva and serum than mice immunized with BBDeCMs ( p < 0.05). After challenge with B. bronchiseptica via the nasal cavity, groups treated with BBDeMCMs or BBDeCMs showed similar patterns with a high survival rate even though there was no significant difference between those groups. These results suggested that mannose moieties in the MCMs enhanced immune-stimulating activities through mucosal delivery due to a specific interaction between mannose groups in the MCMs and mannose receptors on the macrophages.