The popliteal lymph node response to streptozotocin is under type 1, MHC class-I restricted, CD8+ T-cell control
✍ Scribed by G Choquet-Kastylevsky; R Tedone; M.-T Ducluzeau; J Kehren; J.-F Nicolas; J Descotes
- Book ID
- 104324339
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 276 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0300-483X
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✦ Synopsis
The popliteal lymph node (PLN) assay has been proposed to predict the 'autoimmunogenic' potential of xenobiotics. A better understanding of the processes involved in PLN responses is needed to establish the value of this assay for preclinical safety evaluation. In order to determine whether PLN responses involve CD4 + or CD8 + T-cells, the effects of streptozotocin (STZ), a prototypic immunotoxic compound, were analyzed after injection into the hind footpad of C57 BL/6 mice and major histocompatibility complex (MHC) class I or II deficient mice. The involvement of type 1 or type 2 cell control on the production of cytokine mRNAs was analyzed in lymph node cells by quantitative RT-PCR, together with the analysis of a wide range of cytokine mRNAs after STZ injection (