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The polypeptide chain growth rate in amino acid-starved Escherichia coli determined by a novel method

โœ Scribed by Randall Cassada; Hans Matzura


Book ID
115746240
Publisher
Elsevier Science
Year
1976
Weight
675 KB
Volume
418
Category
Article
ISSN
0005-2787

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A method to determine the relative rates of stable and unstable RNA synthesis is presented. The method employs the antibiotic rifampicin. For exponential phase Escherichia coli B/r growing at rates between 0.67 and 2.10 doublings per hr (3.2-fold range in growth rate), the fraction of the instantane