The polymorphism of desialyzed α2HS-glycoptein (AHS): isoelectric focusing in 2.5Murea as a method for identification of genetic variants

✍ Scribed by K. Umetsu; I. Yuasa; T. Suzuki

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 217 KB
• Volume: 73
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00279104

✦ Synopsis

Desialyzed plasma specimens were phenotyped for alpha 2HS-glycoprotein (AHS) using polyacrylamide isoelectric focusing (IEF) in the range pH 5-6 in 2.5 M urea, followed by immunoblotting. The technique used in this study is easy to perform and can differentiate the gene products of all the currently known variants of alpha 2HS-glycoprotein except for AHS 4.