The polymeric p-quinone as a matrix for enzyme immobilization

✍ Scribed by N. P. Ponnuchamy; M. N. Gupta

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 384 KB
• Volume: 33
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260330719

✦ Synopsis

Numerous natural and synthetic materials have been explored as potential supports for enzyme immobilization. '

Recently, KuCera' has described a simple method of preparation of polymeric p-and o-quinones and their use for the immobilization of ovalbumin and subtilisin. The quinone polymers were found to be quite reactive and could be stored at room temperature with almost total retention of binding efficiency even after a period of two months. However, in the case of subtilisin, the only enzyme used by KuEera, the yield of enzyme activity was only 13.17%. In the present work, we have developed a more simplified procedure for enzyme immobilization on a p-quinone polymer. The matrix has been used for immobilization of ovalbumin and two enzymes, namely acid phosphatase and alkaline phosphatase. With both enzymes, excellent yields of enzyme activity were obtained.