The polarization of fluorescence of DNA stains depends on the incorporation density of the dye molecules
β Scribed by Jeanne L. Uy; Charles L. Asbury; Timothy W. Petersen; Ger van den Engh
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 103 KB
- Volume
- 61A
- Category
- Article
- ISSN
- 0196-4763
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## Synopsis The binding constants for the complex formation of more than twenty ring nitrogenand amino-substituted acridine derivatives with calf thymus DNA were measured by a fluorescence method. DNA quenches the fluorescence of the aminoacridine dyes so long &s both amino hydrogens are not subst
The spcclficlty of fluorometry IS u\cful m the qualitative analysis of a compound without prior szpsration. EIowevcr. in some CBSCS. the fluorcsccncc emission due to two compctmg Processes may take place at clo~ly xparatcd wavcIengths. It 15 suggested that in such d ca5c. the polarlzdtlon spectrum m