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The pig caecum model: A suitable tool to study the intestinal metabolism of flavonoids

✍ Scribed by Samira Labib; Annette Erb; Michael Kraus; Thomas Wickert; Elke Richling

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
850 KB
Volume
48
Category
Article
ISSN
1613-4125

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✦ Synopsis

Abstract

Pig caecum was used under anaerobic conditions to metabolize flavonoids from several classes, i. e., chrysin 1, naringenin 2, quercetin 3, and hesperetin 4. Whereas chrysin 1 was not converted by the pig intestinal flora under the experimental conditions used, naringenin 2 was transformed to 3‐(4‐hydroxyphenyl)‐propionic acid and 3‐phenylpropionic acid. Quercetin 3 was metabolized to phloroglucinol, 3,4‐dihydroxyphenylacetic acid, and 3,4‐dihydroxytoluene. Hesperetin 4 was degraded via eriodictyol to 3‐(3‐hydroxyphenyl)‐propionic acid and phloroglucinol. Structural elucidation of the formed metabolites was performed by high‐performance liquid chromatography – diode array detection (HPLC–DAD) as well as HPLC‐electrospray ionization – mass spectrometry (ESI‐MS (MS)) and high resolution gas chromatography‐mass spectrometry (HRGC‐MS) analyses. The time course of microbial conversion of 2–4 was determined by HPLC‐DAD analysis, revealing slow degradation of 2 and rapid transformation of 3 and 4. The results lead to the conclusion that the pig caecum model is a suitable ex vivo model for studying the intestinal degradation of flavonoids.

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