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The phase diagram of dimyristoyl phosphatidylcholine and chain-perdeuterated distearoyl phosphatidylcholine A deuterium NMR spectral difference study

✍ Scribed by Michael R. Morrow; Rajani Srinivasan; Nestor Grandal


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
790 KB
Volume
58
Category
Article
ISSN
0009-3084

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✦ Synopsis


The phase diagram for mixtures of the lipids dimyristoylphosphatidylcholine (DMPC) and chain perdeuterated distearoyiphosphatidylcholine (DSPC-dTo) is determined using 2H-NMR difference spectroscopy. It is found that the temperature of the solidus line increases monotonically with DSPC-dTo concentration and thus that there is no evidence for peritectic behavior in this mixture. This phase diagram is compared to others that have been determined for DMPC/DSPC mixtures and for mixtures of perdeuterated DMPC (DMPC-d54) with DSPC some of which do display peritectic behavior. The possibility that different isotopic compositions give rise to qualitatively different phase diagrams for this system is discussed.