Protoporphyrin IX (Pp IX) is the main photosensitizer in photochemotherapy with 5-aminolevulinic acid (ALA). Pp IX is photolabile and the present work shows that 70-95% of Pp IX in cells is degraded by clinically relevant light exposures (40-200 J cm 22 at 630 nm). During light exposure a small yiel
The pH dependency of protoporphyrin IX formation in cells incubated with 5-aminolevulinic acid
✍ Scribed by Ø. Bech; K. Berg; J. Moan
- Book ID
- 117468415
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 413 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0304-3835
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Human colon adenocarcinoma cells (WiDr) and Chinese hamster lung fibroblasts cells (V79) were incubated with different concentrations of 5-aminolevulinic acid (ALA), and the production of protoporphyrin IX (PpIX) was studied using several techniques. The amount of PpIX produced per cell increased wi