The pattern of protein synthesis in SV40-infected CV-1 cells

✍ Scribed by Hans Fischer; Klaus Munk

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: French
• Weight: 457 KB
• Volume: 5
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910050104

✦ Synopsis

Abstract

The time sequence of induced proteins after SV40 infection has been studied by polyacrylamide gel electrophoresis. The alteration of the protein pattern has been followed in stained gels of protein preparations and in gels of protein preparations after pulse labelling cells with ^14^C amino acids. The proteins of cell extracts were prepared either by precipitation at 60% ammonium sulfate saturation or by disintegration with 8 M urea. The precipitated protein pattern was altered after infection in correlation with the changes in pattern of incorporated isotopes. New bands and increases in bands present in control cultures were observed. After urea treatment the particulate protein pattern revealed three new peaks at 15 h after infection. The most significant alterations, compared to the uninfected CV‐1 cells, occurred at 32 h post infection. Three complexes, peaks 3–8, 10–13, and 14–17, and peak 23 increased at this time. Protein synthetic activity in these areas was confirmed by the electrophoresis of pulse‐labelled proteins.