The pasteur effect in bone marrow, with particular reference to results obtained by different methods
✍ Scribed by Warren, Charles O.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1942
- Tongue
- English
- Weight
- 921 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0095-9898
No coin nor oath required. For personal study only.
✦ Synopsis
Drl~artiwent of Anatomy, Cornell Univrrsity M r d ~e o l College, S e w Fork City THREE FIOCRES
This investigation was undertaken in the effort to analyze the relationship between respiration and fermentation (glycolysis) in rabbit bone marrow a t lowered oxygen tensions. The term "Pasteur effect'' is used in the broad sense as indicating this relationship between respiration, fermentation and oxygen tension; glycolysis is used in the specific sense of meaning lactic acid formation. Bone marrow provides intercsting material in which to study the Pasteur effect, for it is one of the few tissues of the body in which greater physiological activity is observed at lowered oxygen tensions. It has been known for many years (Barcroft, '25) that the polycpthemia which occurs in persons residing at high altitudes is largely the result of au-mented growth and multiplication of the erythroid cells in the bone marrow, and it has also been demonstrated that it is the lowered oxygen tension, rather than thc lowered atmospheric pressure per se which is responsible for this effect.
' By slight modiflcations, the sidewell terhnique can be. adapted for mnking glycolysis mraaurements on tissue suspensions, but in the premnt experiments separate samples of suspension were iised for the ' ' initial ' ' and ' ' final ' ' lactic acid determinations.