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The partial purification and characterization of thiamine pyrophosphatase from rabbit brain

โœ Scribed by J. R. Cooper; M. M. Kini


Book ID
111167073
Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
197 KB
Volume
19
Category
Article
ISSN
0022-3042

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Purification and partial characterizatio
โœ Rabindra Randhawa; Ramji L. Khandelwal ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 721 KB

Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w