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The parDE Operon of the Broad-host-range Plasmid RK2 Specifies Growth Inhibition Associated with Plasmid Loss

✍ Scribed by Richard C. Roberts; Arne R. Ström; Donald R. Helinski


Book ID
115626548
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
969 KB
Volume
237
Category
Article
ISSN
0022-2836

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The cleavage map of the plasmid RK2 was determined for the five restriction endonucleases EcoRI, HindIII, BamH-I, SalI and HpaI. DNA has been inserted into several of these sites and cloned in Escherichia coli. Efforts to obtain derivatives of RK2 reduced in size by restriction endonuclease digestio