Adaptation of the Bradford protein assay
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Bradford O. Fanger
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Article
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1987
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Elsevier Science
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English
โ 567 KB
A procedure was developed for the quantitation of solubilized proteins using the Bradford assay in the presence of glucopyranoside detergents. These detergents solubilized membranebound proteins with minimal background absorbance at 595 nm. Absorbance at 650 nm was also low, indicating that these de