The Outer Membrane of the Marine Gram-Negative Bacterium Alteromonas addita is Composed of a Very Short-Chain Lipopolysaccharide with a High Negative Charge Density
✍ Scribed by Serena Leone; Antonio Molinaro; Luisa Sturiale; Domenico Garozzo; Evgeny L. Nazarenko; Raisa P. Gorshkova; Elena P. Ivanova; Liudmila S. Shevchenko; Rosa Lanzetta; Michelangelo Parrilli
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 264 KB
- Volume
- 2007
- Category
- Article
- ISSN
- 1434-193X
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✦ Synopsis
Abstract
The complete structure of the lipopolysaccharide isolated from the Gram‐negative marine bacterium Alteromonas addita, type strain KMM 3600^T^ = R10SW13^T^, has been elucidated by means of a combined chemical approach and state‐of‐the‐art NMR and MS analyses. Isolation and characterisation of the lipid A moiety and the core oligosaccharide were pursued separately after either acid or alkaline treatment of the lipopolysaccharide. The structure detected was identified as a novel, highly negatively charged, deep‐rough lipopolysaccharide in which a trisaccharide subunit is connected to a typical lipid A glucosamine backbone. Within the core oligosaccharide, a phosphodiester bridge connects a glucose unit to a heptose residue.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)