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The Orientation and Extent of Gene Transfer in Escherichia coli

✍ Scribed by P. D. Skaar and Alan Garen

Book ID
123671108
Publisher
National Academy of Sciences
Year
1956
Tongue
English
Weight
277 KB
Volume
42
Category
Article
ISSN
0027-8424

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The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of