The organization of the β-globin gene of the bivalve molluscAnadara trapeziaand its evolutionary relationship to other invertebrate and vertebrate globin genes

✍ Scribed by N. T. Nassif; W. K. Glenn; A. G. Mackinlay

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 882 KB
• Volume: 39
• Category: Article
• ISSN: 0022-2844
• DOI: 10.1007/bf00178248

✦ Synopsis

Three pairs of oligonucleotide primers based on partial DNA and amino acid sequences were used in a combination of PCR experiments to amplify the ~-globin gene of the bivalve mollusc Anadara trapezia. The sequence of 2,139 bp presented contains the whole of the 13-globin gene with the exception of the 5' flanking sequence. This gene possesses the three-exon-and-two-intron gene structure typical of vertebrate globin genes but the lengths of the introns (762 bp and 690 bp, respectively) are only approximately half the size of those present in a ~3-variant gene previously characterized from this organism. The encoded amino acid sequence shows two changes when compared to the previously published amino acid sequence.