We have developed a vector system for high-copy-number integration into the ribosomal DNA of the yeast Kluyveromyces lactis. This system is analogous to the pMIRY-system developed for Saccharomyces cerevisiae. Plasmids containing a portion of K. lactis rDNA for targeted homologous recombination, as
The organization of the genes for ribosomal RNA on mitochondrial DNA ofKluyveromyces lactis
β Scribed by Gert S. P. Groot; Nel Harten-Loosbroek
- Publisher
- Springer-Verlag
- Year
- 1980
- Tongue
- English
- Weight
- 297 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0172-8083
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β¦ Synopsis
We have constructed a physical map of Kluyveromyces lactis mtDNA using the restriction enzymes HindII and HindIII. In contrast to Saccharomyces, the genes for the large and small ribosomal RNAs are much closer to each other, being separated by a maximal distance of 2,250 base pairs.
π SIMILAR VOLUMES
1. We have isolated large fragments of the mtDNA of the yeast Saccharomyces carlsbergensis and digested these with restriction endonucleases. The digestion products were separated by electrophoresis in agarose gels. 2. Endonucleases EcoRI, HindII + III, HpaI, HindIII and HapII yield 9, 11, 6, 0 and