The occurrence of the hydrogenase in some blue-green algae

✍ Scribed by G. Eisbrenner; E. Distler; L. Floener; H. Bothe

• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 807 KB
• Volume: 118
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00415727

✦ Synopsis

Several blue-green algae were surveyed for the occurrence of the hydrogenase which was assayed by the oxyhydrogen or Knallgas reaction in the intact organisms. 2n aerobically grown cultures, the reaction was detectable in Anabaena cylindrica, Nostoc mus- corurn and in two Anabaena variabilis species, whereas virtually no activity was observed in Anacystis nidulans and Cyanophoraparadoxa. In these latter two algae, the reaction was, however, found after growth under molecular hydrogen for several days, which drastically increased the activity levels with all the algae tested. In the nitrogen fixing species, the activity of the Knallgas reaction was enhanced when all combined nitrogen was omitted from the media. H2 and hydrogenase could not significantly support the CO2-fixation in photoreduction experiments with all blue-green algae investigated here. Hydrogenase was assayed by the dithionite and methyl viologen dependent evolution of hydrogen and was found to be present with essentially the same specific activity levels in preparations of both heterocysts and vegetative cells from Anabaena cylindrica. Na2S20 4 as well as H 2 supported the CzHz-reduction of the isolated heterocysts. The H2-dependent CzH e- reduction did not require the presence of oxygen but was strictly light-dependent where H z served as an electron donor to photosystem I of these cells. It is concluded that hydrogen can be utilized by two different pathways in blue-green algae.