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The occurrence of lunularic and abscisic acids in plants

✍ Scribed by R.J. Pryce


Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
210 KB
Volume
11
Category
Article
ISSN
0031-9422

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High-performance liquid chromatographic
✍ P.B. Sweetser; A. Vatvars πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 534 KB

A HPLC assay for ABA and bound ABA is described. The method uses conventional acid-base ether extractions for partial cleanup of the plant extracts followed by Sephadex G-25 column fractionation. The ABA is separated and detected by HPLC on a 3 m x 2.1 mm i.d. column of SCX-Zipax with a uv detector.

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Methods are given for the quantitation of the plant stress hormone, abscisic acid (ABA), and its two metabolic precursors, ketone and enolate, that are applicable to all species tested so far. The plant extract is homogenized at neutral pH, hexane-soluble neutrals are extracted and discarded, and th