The occurrence of lunularic and abscisic acids in plants
β Scribed by R.J. Pryce
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 210 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0031-9422
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π SIMILAR VOLUMES
A HPLC assay for ABA and bound ABA is described. The method uses conventional acid-base ether extractions for partial cleanup of the plant extracts followed by Sephadex G-25 column fractionation. The ABA is separated and detected by HPLC on a 3 m x 2.1 mm i.d. column of SCX-Zipax with a uv detector.
Methods are given for the quantitation of the plant stress hormone, abscisic acid (ABA), and its two metabolic precursors, ketone and enolate, that are applicable to all species tested so far. The plant extract is homogenized at neutral pH, hexane-soluble neutrals are extracted and discarded, and th
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