The nucleus in relation to plasmagel structure in amoeba proteus; a pressure-temperature analysis

✍ Scribed by Hirshfield, Henry I. ;Zimmerman, Arthur M. ;Marsland, Douglas

• Publisher: Wiley (John Wiley & Sons)
• Year: 1958
• Tongue: English
• Weight: 284 KB
• Volume: 52
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030520205

✦ Synopsis

Interactions between nucleus and cytoplasm very obviously are important in the cell; and recently there have been quite a number of studies designed to analyze these inter-relationships. For example, Brachet ('54, '55) demonstrated nuclear dependence in regard to the synthesis of several cytoplasmic enzymes; D. Cohen ( '56) showed that the DPN level in the cytoplasm of Amoeba falls off subsequent to enucleation ; and Mazia and Hirshfield ('50) reported nuclear dependence with reference to the uptake of P,, by various cells. Holter and Kopac ('37), on the other hand, found that aminopeptidase activity in the Amoeba is more or less independent of the nucleus.

The purpose of the present study was to ascertain whether or not the nucleus may exert some influence on the formation and maintenance of plasmagel structure in Amoeba protezss. It seems well established (see Marsland, '56) that cytoplasmic gelation in cells generally represents an endothermic process -which might be nucleus-dependent for metabolic energy. Also it is known that the cycle of sol-gel changes, which is so intimately related to locomotion in Amoeba, can be modified