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The nucleotide sequence of the replication control region of the resistance plasmid R1drd-19

✍ Scribed by Stougaard, Peter ;Molin, Søren ;Nordström, Kurt ;Hansen, Flemming G.

Publisher
Springer
Year
1981
Tongue
English
Weight
619 KB
Volume
181
Category
Article
ISSN
0026-8925

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✦ Synopsis

The region of plasmid R 1 containing the replication control genes has been sequenced using the Maxam-Gilbert method. The nucleotide sequence of two small PstI restriction fragments (a total of about 1,000 base pairs) was determined for the wild-type R 1 plasmid as well as for two different copy mutants. It was found that one copy mutant has a single base substitution in the fragment which was recently shown to harbor an important inc/cop gene (Molin and Nordström 1980). Furthermore, the sequence indicates the presence of a structural gene that codes for a polypeptide of size 10,500 daltons. Possible gene products predicted from the nucleotide sequences and their role in replication control are discussed.

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