𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The nuclear architectural protein HMGA1a triggers receptor-mediated endocytosis

✍ Scribed by Wuwei Wu; Wei Wan; Alexander D.Q. Li

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
356 KB
Volume
108
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

High mobility group proteins A (HMGA), nuclear architectural factors, locate in the cell nuclei and mostly execute gene‐regulation function. However, our results reveal that a HMGA member (HMGA1a) has a unique plasma membrane receptor; this receptor specifically binds to HMGA‐decorated species, effectively mediates endocytosis, and internalizes extracellular HMGA‐functionalized cargoes. Indeed, dyes or nanoparticles labeled with HMGA1a protein readily enter Hela cells. Using a stratagem chemical cross‐linker, we covalently bonded the HMGA receptor to the HMGA1a‐GFP fusion protein, thus capturing the plasma membrane receptor. Subsequent Western blots and SDS–PAGE gel revealed that the HMGA receptor is a 26‐kDa protein. Confocal live‐cell microscopic imaging was used to monitor the whole endocytic process, in which the internalized HMGA1a‐decorated species are transported by motor proteins on microtubules and eventually arrive at the late endosomes/lysosomes. Cell viability assays also suggested that extracellular HMGA1a protein directly influences the survival ability of Hela cells in a dose‐dependent manner, implying versatility of HMGA1a protein and its potent role to suppress cancer cell survivability and to regulate growth. J. Cell. Biochem. 108: 791–801, 2009. © 2009 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

The nuclear DNA repair protein Ku70/80 i
The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis
✍ Johan Fransson; Carl A.K. Borrebaeck 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 336 KB

## Abstract To be of therapeutic relevance, a tumor‐associated antigen should be expressed on the surface of neoplastic cells but not, or to a significantly lower extent, on cells of non‐transformed nature. The Ku heterodimer (Ku70/80) is involved in DNA double strand break recognition and repair a

Proteins that mediate the nuclear entry
Proteins that mediate the nuclear entry of the goat uterine estrogen receptor activation factor (E-RAF): Identification of a molecular basis for the inhibitory effect of progesterone on estrogen action
✍ Anitha P. Govind; S. Sreeja; Raghava Varman Thampan 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 259 KB

## Abstract A 66 kDa transport protein, tp66, has been identified as the protein that mediates the nuclear transport of the estrogen receptor activation factor (E‐RAF). Indirect evidence shows that tp66 influences the transport of E‐RAF mainly by recognizing the nuclear localization signals (NLS) o

Fibroblast growth factor receptor-1 medi
Fibroblast growth factor receptor-1 mediates the inhibition of endothelial cell proliferation and the promotion of skeletal myoblast differentiation by SPARC: A role for protein kinase A
✍ Kouros Motamed; David J. Blake; John C. Angello; Benjamin L. Allen; Alan C. Rapr 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 291 KB 👁 1 views

## Abstract The role of the matricellular protein SPARC (secreted protein, acidic and rich in cysteine) in modulation of vascular cell proliferation is believed to be mediated, in part, by its ability to regulate the activity of certain growth factors through direct binding. In this study, we demon