The NIT2 nitrogen regulatory protein of Neurospora: expression and stability of nit-2 mRNA and protein
β Scribed by Ying Tao; George A. Marzluf
- Book ID
- 106006403
- Publisher
- Springer-Verlag
- Year
- 1999
- Tongue
- English
- Weight
- 143 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0172-8083
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The nit-2 gene of Neurospora crassa encodes the major nitrogen regulatory protein which acts in a positive fashion to activate the expression of many different structural genes during conditions of nitrogen limitation. An E. coli-expressed NIT2/beta-Gal fusion protein binds specifically to DNA in vi
NIT2, the major nitrogen regulatory protein of Neurospora crassa mediates nitrogen catabolite derepression of the structural genes which specify enzymes of nitrogen catabolism. The promoter of the structural gene for L-amino acid oxidase, a nitrogen-regulated enzyme, was found to contain two NIT2 bi