The New Active-Coupling-Pattern Tilting Experiment for an Efficient and Accurate Determination of Homonuclear Coupling Constants

✍ Scribed by Wiktor Koźmiński

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 102 KB
• Volume: 134
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1998.1479

✦ Synopsis

A new constant-time COSY experiment which allows an efficient determination of accurate homonuclear coupling constant values is presented. Characteristic features include an improved scheme for homonuclear active-coupling-pattern tilting (ACT) and an arbitrarily scaled shift and coupling information (ASSCI) design of the F1 domain. As a result, simple and easy to interpret tilted cross-peak patterns, even for two-spin systems, are obtained with good sensitivity. The relative spacing of chemical-shift differences and coupling splittings is largely under experimental control. The effectiveness of the spectral region selective variant of the new sequence is demonstrated by a determination of the 3JHN, Halpha couplings in a peptide sample. The multiplet-selective variant is shown to produce good results with a terpene. The superiority of the new ACT scheme is additionally demonstrated by an ACT-J spectrum of the peptide. Copyright 1998 Academic Press.