𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The neuropeptide VIP regulates the expression of osteoclastogenic factors in osteoblasts

✍ Scribed by Emma Persson; Ulf H. Lerner

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
439 KB
Volume
112
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Osteoclast formation is controlled by stromal cells/osteoblasts expressing macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-kB ligand (RANKL), crucial for osteoclast progenitor cell proliferation, survival and differentiation, and osteoprotegerin (OPG) that inhibits the interaction between RANKL and its receptor RANK. Recent data have strongly indicated that the nervous system plays an important role in bone biology. In the present study, the effects of the neuropeptide vasoactive intestinal peptide (VIP), present in peptidergic skeletal nerve fibers, on the expression of RANKL, OPG, and M-CSF in osteoblasts and stromal cells have been investigated. VIP and pituitary adenylate cyclase-activating polypeptide 38 (PACAP-38), but not secretin, stimulated rankl mRNA expression in mouse calvarial osteoblasts. In contrast, VIP inhibited the mRNA expressions of opg and m-csf, effects shared by PACAP-38, but not by secretin. VIP did not affect rankl, opg, or m-csf mRNA expression in mouse bone marrow stromal cells (BMSCs). The effects by VIP on the mRNA expression of rankl, opg, and m-csf were all potentiated by the cyclic AMP phosphodiesterase inhibitor rolipram. In addition, VIP robustly enhanced the phosphorylation of ERK and the stimulatory effect by VIP on rankl mRNA was inhibited by the MEK1/2 inhibitor PD98059. These observations demonstrate that activation of VPAC 2 receptors in osteoblasts enhances the RANKL/OPG ratio by mechanisms mediated by cyclic AMP and ERK pathways suggesting an important role for VIP in bone remodeling.

πŸ“œ SIMILAR VOLUMES

Cortisol regulates the expression of Not
Cortisol regulates the expression of Notch in osteoblasts
✍ Rosa M.R. Pereira; Anne M. Delany; Deena Durant; Ernesto Canalis πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 158 KB

## Abstract Glucocorticoids have important effects on osteoblastic replication, differentiation, and function, and the Notch family of receptors is considered to play a role in osteoblastic cell differentiation. We postulated that cortisol could regulate Notch and Notch ligand expression in osteobl

Growth factor regulation of insulin-like
Growth factor regulation of insulin-like growth factor binding protein-6 expression in osteoblasts
✍ Bari Gabbitas; Ernesto Canalis πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 238 KB πŸ‘ 2 views

Previously we have shown that transforming growth factor b (TGF b) 1, basic fibroblast growth factor (FGF), and platelet-derived growth factor (PDGF) BB inhibit the synthesis of insulin-like growth factor (IGF) II, but their effects on IGF binding protein (IGFBP)-6 in osteoblast cultures are not kno

Regulated expression of syndecan-4 in ra
Regulated expression of syndecan-4 in rat calvaria osteoblasts induced by fibroblast growth factor-2
✍ Shu Jun Song; Simon M. Cool; Victor Nurcombe πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 231 KB

## Abstract Fibroblast growth factor‐2 (FGF2) is a member of a prominent growth factor family that drives proliferation in a wide variety of cell types, including osteoblasts. The binding and signal transduction triggered by these mitogens is dependent on glycosaminoglycan (GAG) sugars, particularl