The neuronal protein Kidins220 localizes in a raft compartment at the leading edge of motile immature dendritic cells
✍ Scribed by Lorena Riol-Blanco; Teresa Iglesias; Noelia Sánchez-Sánchez; Gonzalo de la Rosa; Lucía Sánchez-Ruiloba; Noemi Cabrera-Poch; Ana Torres; Isabel Longo; Julio García-Bordas; Natividad Longo; Alberto Tejedor; Paloma Sánchez-Mateos; José Luis Rodríguez-Fernández
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 382 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Abstract
Kidins220, a protein predominantly expressed in neural tissues, is the first physiological substrate for protein kinase D (PKD). We show that Kidins220 is expressed in monocyte‐derived and in peripheral blood immature dendritic cells (im DC). Immature DC (im DC) migrate onto extracellular matrices changing cyclically from a highly polarized morphology (monopolar (MP) stage) to a morphologically symmetrical shape (bipolar (BP) stage). Kidins220 was localized on membrane protrusions at the leading edge or on both poles in MP and BP cells, respectively. CD43, CD44, ICAM‐3 and DC‐SIGN,and signaling molecules PKD, Arp2/3 were found at the leading edge in MP or on both edges in BP cells, showing an intriguing parallelism between morphology and localization of molecular components on the poles of the motile DC. F‐actin co‐localized and it was necessary for Kidins220 localization on the membrane in MP and BP cells. Kidins220 was also found in a raft compartment. Disruption of rafts with methyl‐β‐cyclodextrin induced rounding of the cells, inhibition of motility and lost of Kidins220 polarization. Our results describe for the first time the molecular components of the polesof motile im DC and indicate that a novel neuronal protein may be an important component among these molecules.