The nerve fiber content of Amblystoma aneurogenic limbs

✍ Scribed by Egar, M. ;Yntema, C. L. ;Singer, M.

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 462 KB
• Volume: 186
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401860112

✦ Synopsis

Abstract

The nerveless state of larval Amblystoma limbs rendered aneurogenic by removal of the embryonic neural tube and crest was examined in silver stained light microscope sections and in sections prepared for electron microscopy. The regenerates of these forelimbs were similarly studied. The sections were compared with those taken from the limb and regenerate of normal animals to which the aneurogenic animal had been parabiosed for survival. Nerve fibers were found in 62% of the “aneurogenic” limbs studied at ultrastructural levels. The number of fibers was less than one‐tenth of that in the normal limb and thus well below the expected level required for regeneration. The number of fibers counted with the electron microscope exceeded those observed with the light microscope, emphasizing the limitations of silver stains for the small unmyelinated axons.