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The nature of pyruvate involved in the enzymic formation of L-lactate in the rabbit-muscle lactate dehydrogenase reaction

โœ Scribed by Herbert J. Fromm

Book ID
114300914
Publisher
Elsevier Science
Year
1965
Weight
208 KB
Volume
99
Category
Article
ISSN
0926-6593

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Automated assays of lactate dehydrogenase (LD) in serum are based on measuring the rate of NADH produced in a reverse LD reaction using lactate and NAD. The observed nonlinearity of LD reaction used in earlier assays performed in phosphate buffers has generally been attributed to the formation of a