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The N-terminal amino acid sequences of Brevibacterium sp. R312 nitrile hydratase

✍ Scribed by R. Duran; C. K. N. Chan Kwo Chion; F. Bigey; Prof. A. Arnaud; P. Galzy

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 608 KB
Volume: 32
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620320104

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✦ Synopsis

Abstract

Nitrile hydratase from Brevibacterium sp. R312 was purified to homogeneity. The isoelectric point was 5.75. The two kinds of subunits were separated by reverse phase HPLC and their N‐terminal amino acid sequences were found to be identical to those of Rhodococcus sp. N‐774 nitrile hydratase.

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