𝔖 Bobbio Scriptorium
✦   LIBER   ✦

THE N-ACETYL-β-d-HEXOSAMINIDASES OF CALF AND HUMAN BRAIN

✍ Scribed by D. Robinson; T. W. Jordan; T. Horsburgh


Book ID
111166972
Publisher
John Wiley and Sons
Year
1972
Tongue
English
Weight
770 KB
Volume
19
Category
Article
ISSN
0022-3042

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Demonstration of β-N-acetyl-D-glucosamin
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K,-cells from Drosophila melanogaster, grown under serum-free conditions, produce two p-hexosaminidases and secrete these enzymes into the medium. The two enzymes were separated by DEAE-exchange chromatography. According to their substrate specificities one enzyme is a f3-N-acetyl-D-glucosarninidase

Physical properties of β-N-acetyl-D-gluc
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Kc-cells from Drosophila produce two different beta-D-hexosaminidases, a beta-N-acetyl-D-glucosaminidase (E.C.3.2.1.30) and a beta-N-acetyl-D-hexosaminidase (E.C.3.2.1.52), which are also secreted into the medium. The Mr of both enzymes is about 126,000 +/- 9,700; the S-values are 8.37 +/- 0.44. Bot