## Abstract It is known that insulin treatment increases the rate of protein synthesis in many cells and tissues and that it causes changes in the distribution of ribosomes between free (FP), cytoskeletal‐bound (CBP) and membrane‐bound polysome (MBP) populations. This paper concerns an analysis of
✦ LIBER ✦
The multi-KH protein vigilin associates with free and membrane-bound ribosomes
✍ Scribed by C. Kruse; D. Willkomm; J. Gebken; A. Schuh; H. Stossberg; T. Vollbrandt; P.K. Müller
- Publisher
- Springer
- Year
- 2003
- Tongue
- English
- Weight
- 288 KB
- Volume
- 60
- Category
- Article
- ISSN
- 1420-682X
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A three-step sequential detergent/salt extraction procedure was used in order to isolate three distinct subcellular fractions containing free (FP), cytoskeletal-bound (CBP) and membrane-bound polysomes (MBP), respectively, from Krebs II ascites cells (Vedeler et al., Mol Cell Biochem 100: 183-193, 1