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The morphology of human immunodeficiency virus (HIV) by negative staining

✍ Scribed by Dr. I. L. Chrystie; June D. Almeida

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
596 KB
Volume
25
Category
Article
ISSN
0146-6615

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✦ Synopsis

We have examined preparations of human immunodeficiency virus 1 (HIV-1) and HIV-2 by negative staining electron microscopy. HIV-2 cultures contained large numbers of 130-200 nm particles containing a 130-nm-long by 30-70 nm-wide core. This core is probably of conical or pear-shaped morphology. Some particles exhibited a short fringe that could be seen to comprise a regular arrangement of repeating subunits when visualised end on. Identical particles were found in HIV-1 cultures but in much lower numbers. Attempts to carry out negative staining immune EM were unsuccessful.

Also detected in both HIV-1 and HIV-2 cultures were small (70-80 run) fringed viruslike particles. The possible significance of these particles is discussed.

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