The molecular weights of yeast ribosomal precursor RNAs
โ Scribed by R. C. Brand; R. J. Planta
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 266 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0301-4851
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โฆ Synopsis
The molecular weights of the predominant rRNA precursors as well as those of 26-S and 17-S mature rRNA from Saccharomyces carlsbergensis were determined by polyacrylamide gel electrophoresis in the presence of formamide. Mature 26-S + 5.8-S rRNA was found to have a molecular weight of 1.24 โข 106 while their immediate precursor, 29-S RNA, had a molecular weight of 1.52 โข 106 . Values of 0.70 โข 106 and 0.82 โข 106 were obtained for the molecular weights of mature 17-S rRNA and its 18-S precursor. Finally the 37-S precursor, common to both 29-S and 18-S RNA, was found to have a molecular weight of 2.80 X 106 . Each precursor rRNA, therefore, contains extra sequences not found at the next stage of maturation.
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