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The mitochondrial genome ofSchizosaccharomyces pombe

✍ Scribed by Filomena Manna; Domenica Rita Massardo; Luigi Giudice; Aniello Buonocore; Anno Giulia Nappo; Pietro Alifano; Bernd Schäfer; Klaus Wolf

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
608 KB
Volume
19
Category
Article
ISSN
0172-8083

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✦ Synopsis

The open reading frame of the first intron of the mitochondrial coxl gene (coxlll) was expressed in Escherichia coli. The putative intron-encoded protein stimulated the formation of intra-chromosomal lac+-recombinants about threefold. No stimulation was found when the reading frame was inserted in the opposite direction, or when it was interrupted by a deletion. The intronic open reading frame did not complement recAor recB-mutants of E. coli. In S. pombe, elimination of this intron did not abolish homologous recombination in mitochondria. A possible role of the recombinase activity in yeast mitochondria will be discussed.

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