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The mitochondrial genome of safflower: Isolation and restriction fragment analysis of DNA from CMS and restorer lines

✍ Scribed by Aniko Pay; Marvin A. Smith


Publisher
Springer
Year
1991
Tongue
English
Weight
421 KB
Volume
100
Category
Article
ISSN
0300-8177

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✦ Synopsis


Mitochondria were isolated and purified from paired lines of safflower (Carthamus tinctorius L.) restorer and cytoplasmic male sterile plants using isopycnic gradient centrifugation in isoosmotic Percoll. Agarose gel electrophoresis of restriction endonuclease digested DNAs showed characteristic polymorphism. Restriction fragments representing about 75% of the mitochondrial genome were common to both the fertile and CMS plants, but differed significantly in stoichiometric amounts. The remaining 25% could be accounted for by unique restriction fragments observed in only one or the other plant types.


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