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The mink gene for the λ light immunoglobulin chain: Characterization of cDNA and chromosomal localization

✍ Scribed by T. M. Khlebodarova; N. M. Matveeva; O. L. Serov; A. M. Najakshin; E. S. Belousov; S. V. Bogachev; O. K. Baranov

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
530 KB
Volume
2
Category
Article
ISSN
0938-8990

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✦ Synopsis

A cDNA library from mink spleen was constructed by use of the phage hgtll. The library was screened using polyvalent serum raised against the mink immunoglobulin k chain. As a result, several clones expressing mink immunoglobulin h light chains were identified. Sequencing of one of the clones with an 803 bp insert was performed. The insert comprised nearly the entire coding region for the mature k light immunoglobulin gene with the exception of the leader polypeptide and several amino acids of the FR1 region of the V segment. Compared with the rabbit, mouse and human h light immunoglobulin genes, the homology of the cloned sequence was found to be highest relative to the rabbit gene. With the cloned mink cDNA containing the C-region only as a probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of this mink cDNA sequence and mink chromosomes in the mink-Chinese hamster clone panel allowed us to assign the gene for the k light immunoglobulin constant polypeptide (IGLC) to mink Chromosome (Chr) 4.

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