The microdetermination of cholesterol in serum

✍ Scribed by Herbert K. Alber; J.T. Bryantdetails

• Book ID: 104132449
• Publisher: Elsevier Science
• Year: 1940
• Tongue: English
• Weight: 403 KB
• Volume: 229
• Category: Article
• ISSN: 0016-0032
• DOI: 10.1016/s0016-0032(40)90931-0

✦ Synopsis

The analytical details of cholesterol determination in blood serum have been studied carefully in order to supply reliable data by biochemical methods.

In reviewing the extensive literature, a search was made for a method in which the cholesterol could be isolated as digitonide free from by-products which would interfere in the colorimetric estimation, small amounts of free and total cholesterol could be determined accurately, and serial analyses could be carried out without too much special equipment not ordinarily available. The method finally decided upon was essentially that of Schoenheimer and Sperry (J. Biol. Chem., xo6, 745 (1934)) and G. O. Brun (" Cholesterol Content of the Red Blood Cells in Man." H. K. Lewis and Co. Ltd., London, 1939) with modifications, some of which had been suggested by Fitz (J. Biol. Chem., IO9, 523 (1935)) and some of which have been confirmed by Sperry in the meantime (private communication; Amer. J. Clin. Pathol., 8, 9I (I938)).

The modified method used in this laboratory is described in detail, with some of the modifications from the original procedures being discussed, because it is believed that this method offers some advantages over other routine methods.