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The methyl groups in ribosomal RNA from Escherichia coli

โœ Scribed by Y. Hayashi; S. Osawa; K. Miura

Book ID
115742189
Publisher
Elsevier Science
Year
1966
Weight
744 KB
Volume
129
Category
Article
ISSN
0005-2787

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Ribosomal RNA synthesis has been investigated in an in vitro system consisting of purified E. coli RNA polymerase and phi 80d3 DNA carrying rrnC operon. rRNA comprises about 25% of the total RNA synthesized in this system under optimal conditions and is stimulated by a crude protein fraction prepare

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Nucleoids were isolated from Escherichia coli B/r cells in steady-stage growth at different rates. The number of RNA chains growing on each nucleoid was estimated from the amount and size of RNAs synthesized by endogenous RNA polymerase. This figure represents the number of RNA polymerase molecules