The mechanism of dextransucrase action: Direction of dextran biosynthesis
β Scribed by John F. Robyt; Barbara K. Kimble; Timothy F. Walseth
- Book ID
- 115702217
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 595 KB
- Volume
- 165
- Category
- Article
- ISSN
- 0003-9861
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Germaine et al. [1][2][3] showed that the addition of dextran to Streptococcus mutans dextransucrase digests increased the rate of dextran synthesis. They found that the rate was dependent on the size of the dextran chain and reached a maximum when the average size of the added dextran was 30 glucos
The formation of dextran from sucrose can proceed by two reaction pathways, both of which are catalyzed by dextransucrase (EC 2.4.1.5). One pathway involves chain elongation via sequential transfers of D-glucosyl groups to nonreducing positions of acceptor saccharides' 4. The second pathway, which o
## Abstract Dextransucrase from __Leuconostoc mesenteroides__ was produced in a semicontinuous culture with slow addition of a concentrated sucrose solution. The resulting high activity of the fermentation broth allowed a oneβstep purification method, by gel permeation chromatography (GPC) in 96.4%