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The mechanism of cell division. Hydrostatic pressure effects upon dividing egg cells

✍ Scribed by Marsland, Douglas Alfred

Publisher
Wiley (John Wiley & Sons)
Year
1939
Tongue
English
Weight
715 KB
Volume
13
Category
Article
ISSN
0095-9898

No coin nor oath required. For personal study only.

✦ Synopsis

1 wish to thank Dr. Rheinhard Dohrn for his kindness and interest during my work at the Stazione Zoologica in Naples, and the RockefelIer Institute for the privilege of asing the Jacques Loeb Memorial table at the Naples station.

15

'As is usual in such experiments, 0.95 molal suerose solution, mixed with the sea water of the egg suspension, was used t o provide ail approximately isopychnotic centrifuge medium. In this case, however, 2 parts of the sucrose solution to 1 part of sea water were used, providing a medium slightly hypopycnotic. Thus, although all the eggs were thrown to the Centrifugal end of the chamber, undue crowding and centrifugal compression was avoided.

This force was obtained from an electrically driven centrifuge rotating 15,000 r.p.m. The radius of the centrifuge-pressure bomb, which served as the head, was 6.55 em.

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