✦ LIBER ✦

The Mechanical Properties of Single Chromatin Fibers Under Tension

✍ Scribed by S.H. Leuba; J. Zlatanova; M.A. Karymov; R. Bash; Y.-Z. Liu; D. Lohr; R.E. Harrington; S.M. Lindsay

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 878 KB
Volume: 1
Category: Article
ISSN: 1438-5163
DOI: 10.1002/1438-5171(200006)1:2<185::aid-simo185>3.0.co;2-8

No coin nor oath required. For personal study only.

✦ Synopsis

An atomic force microscope was used to image and stretch single synthetic chromatin fibers consisting of twelve core nucleosomes with no linker histones. Peaks in the forcecurves are attributed to sequential detachment of nucleosomes from the glass support. The short distances between peaks and reversibility of the pulling process show that the nucleosomes remain intact even at tensions on the order of 350 picoNewtons (pN). This is more than an order of magnitude larger than the force required to de-spool histone octamers from the nucleosomal DNA in laser optical tweezer measurements made with longer molecules, suggesting that loading rates and the length of the molecule are important factors in determining the force required to break inter-molecular bonds.

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