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The measurement of ubiquitin and ubiquitinated proteins

✍ Scribed by Edward G. Mimnaugh; Paolo Bonvini; Len Neckers

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
131 KB
Volume
20
Category
Article
ISSN
0173-0835

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## Abstract This article describes an undergraduate biochemistry laboratory investigating the ubiquitin‐proteasome pathway in yeast. In this exercise, the enzyme β‐galactosidase (β‐gal) is expressed in yeast under the control of a stress response promoter. Following exposure to heat stress to induc