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The measurement of the main PGE metabolite, 13,14-dihydro-15-keto prostaglandin E by radioimmunoassay using methyl oxime stabilization

โœ Scribed by Kelly, R.W.; Gallacher, A.F.; Johnston, T.A.; Harrison, N.W.


Book ID
122069953
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
576 KB
Volume
45
Category
Article
ISSN
0952-3278

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The measurement of 13,14-dihydro-15-keto
โœ R. W. Kelly; M. H. Abel ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 360 KB ๐Ÿ‘ 1 views

The main metabolite of prostaglandin E, (PGE2) 13,14-dihydro-Sketo-PGE, readily dehydrates and can subsequently form a cyclic derivative. This problem can be overcome by the immediate formation of oximes of the 9 and 15 ketones in aqueous solution followed by subsequent extraction, methylation and t