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The measurement of enzyme-ligand dissociation constants by relaxation kinetics

✍ Scribed by Alan D.B. Malcolm


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
138 KB
Volume
77
Category
Article
ISSN
0003-2697

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The Ligand Affinity of Proteins Measured
✍ Dennis E. Epps; Ronald W. Sarver; Joseph M. Rogers; John T. Herberg; Paul K. Tom πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 136 KB

An isothermal denaturation kinetic method was developed for identifying potential ligands of proteins and measuring their affinity. The method is suitable for finding ligands specific toward proteins of unknown function and for large-scale drug screening. It consists of analyzing the kinetics of iso