Meiosis in diploids of Saccharomyces cerevisiae either homozygous or heterozygous for the dmt gene result, in about 5% of the meiotic products, in spores which have undergone an interconversion at the mating-type locus. Some of these interconversions appear to be the result in the generation of spon
The mating reaction in yeast
โ Scribed by Blamire, John ;Melnick, Lawrence M.
- Book ID
- 104727252
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 637 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
The isolation and preliminary characterisation of a mutation, not linked to the mating type locus, but which apparently alters the mating type directed sequence of events during sexual conjugation is described. Haploids of the alpha mating type carrying this gene will now mate with other alpha haploids, creating diploids homozygous for the alpha mating type locus. This gene can be carried, but not expressed, in a haploids, however in a/alpha diploids homozygous for this gene mating is now possible with both a and alpha haploids giving either a/a/alpha or a/alpha/alpha triploids. Using these strains mating-deficient mutants have been isolated and preliminary results on their characterisation presented.
๐ SIMILAR VOLUMES
Mating in Yarrowia lipolytica can be induced by mixing cells of both complementary mating types (A and B) in yeast extract-malt-medium. By means of an inbreeding programme, strains with significantly improved mating frequencies were selected. The main events of the mating process, that have been det
Crosses involving the partially defective mating type mutant B102 (functional in conjugation, defective in meiosis) have confirmed the notion that, in Schizosaccharomyces pombe, certain mating type mutations can arise by transposition. A copy of the mat2P segment (specifying + mating type) is transp