The MAP kinase JNK-1 of Caenorhabditis elegans: Location, activation, and influences over temperature-dependent insulin-like signaling, stress responses, and fitness
✍ Scribed by Marc Wolf; Frank Nunes; Arne Henkel; Alexander Heinick; Rüdiger J. Paul
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 375 KB
- Volume
- 214
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
The mitogen‐activated protein kinase (MAPK) pathways and insulin‐like signaling play pivotal roles in cellular stress response. Using an anti‐phospho‐SAPK/JNK antibody and a daf‐16::GFP‐based reporter assay, the present study shows in Caenorhabditis elegans that ambient temperature (1–37°C) specifically influences the activation (phosphorylation) of the MAP kinase JNK‐1 as well as the nuclear translocation of DAF‐16, the main downstream target of insulin‐like signaling. Activated JNK‐1 was detected only in neuronal cells, and JNK‐1 was found to be controlled by the MAPK JKK‐1 under heat stress. Comparative analyses on the wildtype and a jnk‐1 deletion mutant revealed a promoting influence of JNK‐1 on both nuclear DAF‐16 translocations and DAF‐16 target gene (superoxide dismutase 3, sod‐3) expressions within peripheral, non‐neuronal tissue. Consequently, the mutant exhibited a reduced thermal tolerance and reproductive fitness at higher temperatures. These results provide evidence of indirect interactions between neuronal MAPK and peripheral insulin‐like signaling in response to environmental stimuli (temperature, H~2~O~2~). J. Cell. Physiol. 214: 721–729, 2008. © 2007 Wiley‐Liss, Inc.