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The many routes of bacterial transfer RNAs after aminoacylation

โœ Scribed by Sylvain Blanquet; Yves Mechulam; Emmanuelle Schmitt


Book ID
114314846
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
446 KB
Volume
10
Category
Article
ISSN
0959-440X

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โœ M.X. Wu; K.A.W. Hill ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 272 KB

A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as