The luminescent bacteria toxicity test: Its potential as anin vitro alternative
✍ Scribed by Bulich, Anthony A. ;Tung, Ker-Kong ;Scheibner, Grace
- Publisher
- John Wiley and Sons
- Year
- 1990
- Weight
- 467 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0884-3996
No coin nor oath required. For personal study only.
✦ Synopsis
During the past several years, the use o f animals for toxicity testing has come under critical surveillance. For ethical and economic reasons, various techniques have been developed and proposed as potential alternatives for some of the whole animal toxicity assays. One assay proposed as an alternative t o animal testing is the luminescent bacteria toxicity test (LBT), provided under the trade name o f MicrotoxQ. The sensitivi t y and specificity o f the LBT was compared with t w o commonly used toxicity tests-the L-929 Minimal Eagle's Medium (MEM) elution cytotoxicity test and the Draize test. Cytotoxicity and LBT test data f r o m 709 medical device and biomaterial extracts were compared using a positive/negative ranking system which provided a measurement o f false positive and false negative results. These data were compiled from nine separate laboratories producing or using a wide variety o f biomaterials and medical device products. The LBT was more sensitive than the tissue culture assay and displayed f e w false negatives. LBT EC, , values were compared with eye irritancy categories for a group o f 34 chemicals and 27 personal care products. As with tissue culture, the LBT was more sensitive and produced minimal false negatives. The data from this study indicate the LBT has potential as a rapid, simple method t o screen biomaterials and personal care products for toxicity and irritancy.