The localized incorporation of3H-L-fucose into cell-wall polysaccharides of the cap and epidermis of corn roots
✍ Scribed by E. G. Kirby; R. M. Roberts
- Publisher
- Springer-Verlag
- Year
- 1971
- Tongue
- English
- Weight
- 970 KB
- Volume
- 99
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
When aH-L-fueose is provided to corn roots, a large proportion of the radioactivity is recovered in the polysaccharides extracted from the cell wall. Hydrolysis of this material yields aH-L-fucose as the sole radioactive product. Two metabolites, identified tentatively as L-fucose-l-phosphate and a nucleoside allphosphate derivative of L-fucose have been isolated from the ethanol soluble fractions of the roots and are possibly precursors of the polysaccharide. Autoradiographs of tissue sections indicate that the synthesis of polysaccharides containing L-fucose is confined largely to the root-cap and epidermis. The outer epidermal wall and root-cap slime are particularly radioactive and, therefore, likely to be relatively rich in fucose. By contrast the cell walls from more deeply lying tissues incorporate negligible amounts of the sugar.